Sheep Tissue Acetyl Coenzyme A-Dependent Arylamine N-Acetyltransferases
β Scribed by Tulin Guray; Tugba Guvenc
- Book ID
- 117567448
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 291 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0742-8413
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π SIMILAR VOLUMES
The raw extracts of a series of microorganisms were screened for the presence of acetyl-coenzyme A: arylamine N-acetyltransferase (AAAT) using a radioactive assay with 3H-acetyl-coenzyme A and aniline as substrates. Enzyme activities were primarily detected in the soluble fractions of Bacillus and N
Simple and sensitive spectrophotometric and radiochemical procedures are described for the assay of acetyl-CoA:arylamine N-acetyltransferase (NAT; EC 2.3.1.5), which catalyzes the reaction acetyl-CoA + arylamine----N-acetylated arylamine + CoASH. The methods are applicable to crude tissue homogenate