Shadow promoters in the F plasmid transfer operon
โ Scribed by Fowler, Tim ;Thompson, Russell
- Book ID
- 104726768
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 554 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
โฆ Synopsis
The in vivo transcription start site for PYZ, the promoter of the F plasmid transfer operon, has been located. When transcription start sites for PYZ are deleted, two additional shadow promoters, upstream of PYZ, are activated in vivo. The shadow promoters correspond to those previously identified by in vitro run-off transcription experiments. The activated shadow promoters are stimulated by TraJ protein, a positive regulator of the transfer operon.
๐ SIMILAR VOLUMES
The nucleotide sequence of the DNA encoding the traM, finP and the promoter proximal segment of the traJ gene of the F plasmid has been determined. The predicted amino acid sequence for the traM protein shows that this inner membrane protein contains no signal sequence. The promoters for both the tr