SFE kinetics of bioactive compounds from Helianthus annuus L.
✍ Scribed by Lourdes Casas; Casimiro Mantell; Miguel Rodríguez; Ascensión Torres; Francisco A. Macías; Enrique J. Martínez de la Ossa
- Book ID
- 102924770
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 819 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
The kinetics of the supercritical fluid extraction of bioactive compounds from sunflower using CO~2~ as solvent were studied in order to establish an efficient method for this extraction. The influence of time of extraction at different solvent flow rates was investigated. The extraction pressure and temperature were optimized in previous studies and these values used were 400 bar and 50°C. The extraction yields and the bioactivity levels of the extracts were also analysed. The results indicate that the most appropriate extraction time is between 120 and 180 min depending on the solvent flow and the pretreatment of the sample. The dried sample extracted at 40 g/min, the congealed sample extracted at the same flow and 180 min, and the dried sample extracted at 25 g/min for 120 min showed the best activity profiles. The extractions were carried out in a pilot plant with an extraction vessel with a capacity of 2 L.
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The structure of helianthosid 2 was reexamined by a spectroscopic investigation using one-and two-dimensional NMR spectroscopy and all 'H and "C signals of the saccharide part were assigned. NOE difference and ROESY experiments allowed a conformational analysis of the orientation of the monosacchari
The glycerol 3-phosphate acyltransferase (GPAT, EC 2.3.1.15) from sunflower (Helianthus annuus L.) microsomes has been characterised and partially purified. The in vitro determination of activity was optimized, and the maximum value for GPAT activity identified between 15 and 20 days after flowering