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Serum-free medium for long-term growth, freezing, cloning and fusion of myelomas and hybridomas

✍ Scribed by Ji-Liang Li; Ying-Jie Li; Sui Chao; Ming-Hui Ouyang; Yi-Bing Peng


Book ID
112801579
Publisher
Society for In Vitro Biology
Year
1993
Tongue
English
Weight
162 KB
Volume
29
Category
Article
ISSN
1475-2689

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Development of a serum-free medium for g
✍ Tomoyuki Kawamoto; J.Denry Sato; Anh Le; Don B. McClure; Gordon H. Sato πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 771 KB

In this study, we have devised a hormone-supplemented, lipid-enriched serum-free medium, designated KSLM, that supports the growth of NS-1 mouse myeloma cells and have studied its applicability to the efficient isolation of antibody-producing hybridomas formed from the fusion of NS-1 myeloma cells a