Serum-free medium for hybridoma and parental myeloma cell cultivation: A novel composition of growth-supporting substances

Development of a serum-free medium for g

Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas

✍ Tomoyuki Kawamoto; J.Denry Sato; Anh Le; Don B. McClure; Gordon H. Sato 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 771 KB

In this study, we have devised a hormone-supplemented, lipid-enriched serum-free medium, designated KSLM, that supports the growth of NS-1 mouse myeloma cells and have studied its applicability to the efficient isolation of antibody-producing hybridomas formed from the fusion of NS-1 myeloma cells a