Serum-free culture of primitive murine hemopoietic colony-forming cells
โ Scribed by Paul Baines; Francoise Cormier; Nicole Lucien; Georges A. Boffa
- Book ID
- 112212038
- Publisher
- AlphaMed Press
- Year
- 1986
- Tongue
- English
- Weight
- 629 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1066-5099
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Mouse bone marrow cells in suspension were separated into a number of fractions on the basis of cell density by equilibrium density gradient centrifugation, or on the basis of cell size by velocity sedimentation. After each type of separation, the cells from the various fractions were assayed for th
The requirements for the serum-free culture of PC13 murine embryonal carcinoma cells were determined. Supplementation of a 50:50 mixture of Dulbecco's modified Eagles medium and MCDB104 with transferrin (5 @ n l ) , human high-density lipoprotein (HDL) (100 &$nl), and h u m a n low-density lipoprote