Serum-free culture of PC13 murine embryonal carcinoma cells

✍ Scribed by John K. Heath; M. Jane Deller

• Book ID: 102883439
• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 629 KB
• Volume: 115
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041150302

✦ Synopsis

The requirements for the serum-free culture of PC13 murine embryonal carcinoma cells were determined. Supplementation of a 50:50 mixture of Dulbecco's modified Eagles medium and MCDB104 with transferrin (5 @ n l ) , human high-density lipoprotein (HDL) (100 &$nl), and h u m a n low-density lipoprotein (LDL) (50 pg/ml) supported growth comparable to that observed with 5% foetal calf serum. Media supplementation with lipoproteins apparently substitutes for t h e effects of insulin, desoctapeptide insulin (DOP), or multiplication-stimulating activity (MSA) on EC cell multiplication. Clonal growth of PC13 EC cells in this serum-free medium could only be achieved in the presence of suitable feeder cell monolayers. These observations demonstrate that PC13 EC cells do not have an absolute requirement for exogenous m itogens to support mu It i pl icat ion.