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Serotonin binding sites of human blood platelets

✍ Scribed by Byung K. Kim; Manfred Steiner; Mario G. Baldini

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
540 KB
Volume
106
Category
Article
ISSN
0003-2697

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✦ Synopsis

The possible use of formaldehyde-fixed platelets to characterize and enumerate the specific receptor sites for S-hydroxytryptamine was investigated. Equilibrium, pa-dependent capacity and specificity of Shydroxytryptamine binding by formaldehyde-fixed platelets were demonstrated. Analysis of binding data revealed two different sites: (i) high affinity with low capacity, and (ii) low affinity with high capacity. The results of binding studies using nonfixed control platelets were comparable with those of formaldehyde-fixed platelets. The versatility of formaldehyde fixation for studies of surface receptors was also shown by demonstrating nearly equal binding affinity for PGE, in control and formaldehyde-treated platelets. Our results indicate that formaldehyde fixation is a useful tool for the study of membrane receptor sites especially when active transport of the ligand such as serotonin is a problem.

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