Sequential 1H-NMR assignments and secondary structure of the sea anemone polypeptide anthopleurin-A
β Scribed by Bridget C. MABBUTT; Raymond S. NORTON
- Book ID
- 115127686
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 860 KB
- Volume
- 187
- Category
- Article
- ISSN
- 1432-1327
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The effects of pH and temperature on the 300-MHz 'H-nmr spectra of three cardioactive polypeptides from sea anemones, anthopleurin-A from Anthopleura zunthogrammicu (AP-A) and Ammonia sukutu toxins I and I1 (ATX I and 11), are described. AP-A and ATX I1 exhibit major spectral heterogeneity. Evidence
Recently, a new procedure for the assignment of protein 'H-nmr spectra was introduced that relies on stereochemical considerations of proton-proton distances in polypeptides and on the use of two-dimensional nmr for obtaining 'H-lH through-bond and through-space connectivity maps. In the present pap