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Sequential individual resonance assignments in the 1H-NMR spectra of polypeptides and proteins

✍ Scribed by Kurt Wüthrich

Publisher: Wiley (John Wiley & Sons)
Year: 1983
Tongue: English
Weight: 449 KB
Volume: 22
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360220121

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✦ Synopsis

Recently, a new procedure for the assignment of protein 'H-nmr spectra was introduced that relies on stereochemical considerations of proton-proton distances in polypeptides and on the use of two-dimensional nmr for obtaining 'H-lH through-bond and through-space connectivity maps. In the present paper a particular aspect of this assignment procedure is discussed in more detail, i.e., how to obtain individual resonance assignments from identification of amino acid side-chain spin systems and identification of neighboring residues in the amino acid sequence.

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