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Sequencing of double-stranded polymerase chain reaction products for mutation analysis

โœ Scribed by R.D. Anderson; C.-Y. Bao; D.T. Minnick; J. Baxter; M.L. Veigl; W.D. Sedwick

Book ID
113194434
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
888 KB
Volume
288
Category
Article
ISSN
0027-5107

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A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the