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Sequence-specific detection of nucleic acids utilizing isothermal enrichment of G-quadruplex DNAzymes

✍ Scribed by Hao-Jie Xiao; Ho Chol Hak; De-Ming Kong; Han-Xi Shen

Book ID
116179523
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
563 KB
Volume
729
Category
Article
ISSN
0003-2670

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Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt