An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o
✦ LIBER ✦
Sequence-specific affinity precipitation of oligonucleotide using poly(N-isopropylacrylamide)–oligonucleotide conjugate
✍ Scribed by Takeshi Mori; Daisuke Umeno; Mizuo Maeda
- Book ID
- 101338517
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 164 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
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