Sequence-Dependent Dynamics in Duplex DNA
β Scribed by Okonogi, T.M.; Alley, S.C.; Reese, A.W.; Hopkins, P.B.; Robinson, B.H.
- Book ID
- 118513963
- Publisher
- Biophysical Society
- Year
- 2000
- Tongue
- English
- Weight
- 184 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0006-3495
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Many important applications of DNA sequence-dependent hybridization reactions have recently emerged. This has sparked a renewed interest in analytical calculations of sequence-dependent melting stability of duplex DNA. In particular, for many applications it is often desirable to accurately predict
Melting experiments were conducted on 22 DNA dumbbells as a function of solvent ionic strength from 25-115 mM Na Ο© . The dumbbell molecules have short duplex regions comprised of 16 -20 base pairs linked on both ends by T 4 single-strand loops. Only the 4 -8 central base pairs of the dumbbell stems