To determine the origin of Ogura male sterile cytoplasm in radish (Raphanus sativus L.), wild and cultivated radishes were crossed. Three types of progeny resulted from the F1 hybrids between the wild radish from 'Kushikino' with Ogura-type mtDNA and the cultivars ('Uchiki-Gensuke' or 'Comet'). The
Sequence analysis on the mitochondrialorfBlocus in normal and Ogura male-sterile cytoplasms from wild and cultivated radishes.
โ Scribed by Toru Terachi; Keiko Yamaguchi; Hiroshi Yamagishi
- Book ID
- 106004342
- Publisher
- Springer-Verlag
- Year
- 2001
- Tongue
- English
- Weight
- 138 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0172-8083
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Ogura male-sterile cytoplasm was surveyed in common Japanese radish cultivars and in wild radishes growing in various localities in Japan. Mitochondrial (mt) DNA rearrangement involving the atp6 gene was used as a molecular marker. To detect the mtDNA rearrangement, polymerase chain reactions (PCR)
We have cloned and sequenced the cytochrome oxidase subunit II (coxII) gene from both normal and cytoplasmic male-sterile (CMS) sugar beet. The normal coxII (designated NcoxII) locus was found to be located 1491 bp upstream from the gene for cytochrome oxidase subunit I (coxI) on the same DNA strand