Molecular and biological studies on male sterile cytoplasm in Cruciferae. II. The origin of Ogura male sterile cytoplasm inferred from the segregation pattern of male sterility in the F1progeny of wild and cultivated radishes (Raphanus sativusL.)

✍ Scribed by Hiroshi Yamagishi; Toru Terachi

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 452 KB
• Volume: 80
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00039651

✦ Synopsis

To determine the origin of Ogura male sterile cytoplasm in radish (Raphanus sativus L.), wild and cultivated radishes were crossed. Three types of progeny resulted from the F1 hybrids between the wild radish from 'Kushikino' with Ogura-type mtDNA and the cultivars ('Uchiki-Gensuke' or 'Comet'). The segregation patterns of the male sterility were compared with those of Ogura cytoplasm. The male sterility induced in the Fl hybrid was maintained by crossing with 'Uchiki-Gensuke', that maintains Ogura male sterility. In the two types of progeny, in which 'Comet' (a restorer of Ogura cytoplasm) was used as one of the parents, both fertile and sterile plants segregated at the predicted ratio on the assumption that a single dominant fertility restoring gene exists in the restorer. From these results, we concluded that the Ogura cytoplasm is identical to that of the wild radish, and the former originated in a population of Japanese wild radish.