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Sephadex LH-20 column separation of thyroidal iodoamino acids

โœ Scribed by Albert D. Williams; Donna E. Freeman; Warner H. Florsheim

Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
873 KB
Volume
45
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

A solvent system consisting of low-boiling organic solvents and ammonium hydroxide effectively separated iodoamino acids of the thyroid on Sephadex LH-20 gel filtration columns. The iodoamino acids were eluted from the column in a sequence unrelated to their molecular weights suggesting that adsorptive properties of the dextran gel rather than gel exclusion effects governed the elution volume of the compounds.

The eluted iodoamino acids were recovered in a pure form by low-temperature evaporation of the solvents at reduced pressure. Good recovery of iodoamino acids from the column was obtained and reproducibility of column performance was satis-'factory.

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