Separations of some coumarins of higher plants by liquid chromatography

✍ Scribed by H.Joan Thompson; Stewart A. Brown

• Book ID: 104146679
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 822 KB
• Volume: 314
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97746-5

✦ Synopsis

The behaviour of 67 coumarins occurring in higher plants was examined by high-performance liquid chromatography. Resolutions of neutral coumarins were achieved on a normal-phase silica column (lo-pm particles) in hexaneethyl acetate solvent systems and on a Cis reversed-phase column (5 pm) with aqueous acetonitrile or aqueous methanol. Phenolic coumarins were resolved by stepwise elution with increasing concentrations of aqueous methanol containing acetic acid, sometimes with the addition of tetrahydrofuran, on the reversed-phase column. A mixture of ten coumarins simulating the neutral coumarin fraction of Citrus aurantifoliu was fully resolved by the use of the two columns in sequence.