Separations of Open-Circular DNA Using Pulsed-Field Electrophoresis
โ Scribed by Stephen D. Levene and Bruno H. Zimm
- Book ID
- 123634434
- Publisher
- National Academy of Sciences
- Year
- 1987
- Tongue
- English
- Weight
- 774 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/29915
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It has been reported that secondary pulsed field gel (SPFG) electrophoresis can dramatically increase the speed of separation of large DNA molecules without a decrease in resolution (Zhang, T. Y., Smith, C. L., and Cantor, C. R. (1991) Nucleic Acids Res. 19, \(1291-\) 1296). However, our attempts to
A summary of the three main one-dimensional pulsed-field strategies (zero-integrated field, forward-biased field, and high frequency modulation) used for separating DNA molecules without band inversion within a preselected size range is given. Each of these strategies has size-specific features whic