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Separation of wide-range DNA fragments using non-gel capillary electrophoresis

โœ Scribed by Zhao Tao; Liu Qiping; Cheng Jieke


Book ID
105623672
Publisher
Wuhan University
Year
1998
Tongue
English
Weight
51 KB
Volume
3
Category
Article
ISSN
1007-1202

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## Abstract Polyacrylamide gels are unstable in 50โ€ฮผm internal diameter capillaries at electric fields greater than about 500 V cm^โˆ’1^ due to the formation of bubbles, which destroy the separation. We describe the use of a modified polyacrylamide capillary gel that is stable at electric fields of a