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Effect of temperature on the separation of DNA restriction fragments in capillary gel electrophoresis

✍ Scribed by Andras Guttman; Nelson Cooke


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
570 KB
Volume
559
Category
Article
ISSN
1873-3778

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## Abstract Polyacrylamide gels are unstable in 50‐μm internal diameter capillaries at electric fields greater than about 500 V cm^βˆ’1^ due to the formation of bubbles, which destroy the separation. We describe the use of a modified polyacrylamide capillary gel that is stable at electric fields of a