Separation of the methylated nucleoside constituents of ribonucleic acid by two-dimensional thin-layer chromatography

✍ Scribed by Theodore W. Munns; Harold F. Sims

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 489 KB
• Volume: 111
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)99290-2

✦ Synopsis

In an attempt to separate a number of methylated nucleoside constituents reportedly present in various mammalian RNA preparations, a comparison of the migration of selected nucleoside standards was assessed via thin-layer chromatographic techniques with respect to three solvent systems and an adsorbent of varying proportions of silica gel and microcrystalline cellulose. The results of these studies revealed that a mixture of seventeen methylated constituents could be separated following two-dimensional development on an adsorbent containing CO';/, cellulose and 40% silica gel. Constituents separated included the 2'-0-methylated derivatives of adenosine.

guanosine, cytidine and uridine, I-, Nh-and N".N"-methyladenosines, Imethylinosine, I -, 7-, NZ-and NZ.NZ-methylguanosines, 3-, 4-and 5-methylcytidines and 3-and 5-methyluridines.

In addition, all of the above constituents were separated from their respective non-methylated nucleosides and bases.