Separation of tetracycline and its related substances by capillary zone electrophoresis

✍ Scribed by Van Schepdael, Ann ;Saevels, Jan ;Lepoudre, Xavier ;Kibaya, Rukia ;Gang, Ni Zhi ;Roets, Eugèn ;Hoogmartens, Jos

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 337 KB
• Volume: 18
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240181104

✦ Synopsis

Abstract

Tetracycline was separated from its main impurities 4‐epitetracycline, anhydrotetracycline, 4‐epianhydrotetracycline, chlortetracycline, and demethyltetracycline by capillary electrophoresis. Systematic method development was performed in which following parameters were consecutively optimized: type and pH of the buffer, buffer concentration, type and concentration of organic modifier, voltage and temperature. Qualitative and quantitative aspects of the method are compared with liquid chromatography.