Separation of sugars by h.p.l.c. on copper silicate gel
✍ Scribed by François Guyon; Alain Foucault; Marcel Caude; Robert Rosset
- Book ID
- 102641381
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 227 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0008-6215
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TLC separation of glucose, maltose, lactose, sorbitol and sucrose on silica gel plates impregnated with transition metal ions, Cu(II), NI(II), Zn(I1) or Cd(II), has been achieved. The identification is very distinct by using KMnO, (0.5%) in 0.1 M NaOH as the spray reagent. Two new solvent systems, (
Sugars were separated gas chromatographically on short apolar glass capillary columns by using cold, on-column injection (OCI) techniques. After silylation, oligomers up to the hexasaccharides could be efficiently separated in reasonable retention times. Response factors of silylated sugars were det