𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation of specific antibodies to tstas of adeno 12 and polyoma tumors

✍ Scribed by Hans O. Sjögren; Dan Motet

Book ID
102274475
Publisher
John Wiley and Sons
Year
1969
Tongue
French
Weight
423 KB
Volume
4
Category
Article
ISSN
0020-7136

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✦ Synopsis

Anfisera specifically directed against the TSTAs qf' adeno I 2 and polyoma nroitse tumors were absorbed with cells of other tumors induced by the same viruses. The antibodies adsorbed on tumor cells were subsequently eluted at p H 3. The eluate, absorbed serum and original serum were tested by the colony inhibition ( C I ) tcwhnique for coniplentent dependent cytotoxicity against adeno 12 and polyoma tumor cells syngeneic with the serum donors. Similar arlsorptioti-elrrtion experiments were performed with crude cell membrane fractions.

It was demonstrated that the anti-tumor antibody activity of the original serum can be absorbed away by both intact tumor cells and crude cell membrane fractions. The specific antibodies can subsequently be recovered by elution (at low p H ) jiom the cells used for absorption.

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