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Separation of sialyl-oligosaccharides by medium pressure anion-exchange chromatography on mono Q®

✍ Scribed by Johannes van Pelt; Jan B.L. Damm; Johannis P. Kamerling; Johannes F.G. Vliegenthart

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
629 KB
Volume
169
Category
Article
ISSN
0008-6215

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✦ Synopsis

On columns prepacked with the recently introduced anion-exchange material, Mono Q (Pharmacia), sialyl-oligosaccharides could be fractionated excellently according to the sialic acid content. With u.v. absorption at 214 nm as the detection method, analytical runs of carbohydrate material on the microgram scale, were possible. The value of the method for preparative purposes was demonstrated for sialic acid-containing carbohydrates obtained from human serotransferrin by hydrazinolysis.

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