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Separation of proteins via semicontinuous pH parametric pumping

✍ Scribed by H. T. Chen; T. K. Hsieh; H. C. Lee; F. B. Hill

Publisher
American Institute of Chemical Engineers
Year
1977
Tongue
English
Weight
738 KB
Volume
23
Category
Article
ISSN
0001-1541

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✦ Synopsis

Abstract

A semicontinuous __p__H parametric pump for separating proteins was experimentally investigated using the model system haemoglobin and albumin on a Sephadex ion exchanger. The pump considered had a center feed between an enriching column and a stripping column and was operated batchwise during upflow and continuously during downflow. Various factors affecting separations were examined. It was shown that parametric pumping is capable of separating proteins with high separation factors.

πŸ“œ SIMILAR VOLUMES

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