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Separation of proteins via multicolumn pH parametric pumping

✍ Scribed by H. T. Chen; W. T. Yang; Ura Pancharoen; Robert Parisi

Publisher
American Institute of Chemical Engineers
Year
1980
Tongue
English
Weight
927 KB
Volume
26
Category
Article
ISSN
0001-1541

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✦ Synopsis

Abstract

Fractionation of protein mixtures by multicolumn __p__H parametric pumping is investigated theoretically and experimentally. The parapumps considered consist of a series of columns packed alternately with cation and anion exchangers. Various methods of operation of the parapumps are discussed. Two separation problems are examined: enrichment and splitting. Experimental data were obtained for two‐column systems and compared with the calculated results based on an equilibrium theory.

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