Separation of phosphorylated nucleoside reverse transcriptase inhibitors by capillary electrophoresis

✍ Scribed by Melissa N. Dunkle; Jill Hochreister; Robin DiFrancesco; Gene D. Morse; Luis A. Colón

• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 335 KB
• Volume: 93
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2009.04.001

✦ Synopsis

Capillary electrophoretic (CE) conditions were established to separate multiple nucleoside reverse transcriptase inhibitor triphosphates (NRTI-TPs). These are the active drug forms of nucleoside reverse transcriptase inhibitors (NRTIs) used to treat HIV-infected patients and two NRTIs are always used as part of a three or four drug regimen. Two buffer systems were suitable to separate multiple NRTI-TPs using CE. A carbonate/borate buffer under a positive applied voltage provided for the separation of six different NRTI-TPs of interest. A phosphate buffer, however, under suppressed electroosmotic flow conditions (i.e., low pH) and a negative applied voltage proved to be more effective than the carbonate/borate system. Addition of EDTA to the run buffer improved resolution and overall peak shape of the NRTI-TP. The established experimental conditions can be used to separate multiple NRTI-TPs in peripheral blood mononuclear cells (PBMCs) from human whole blood.