Separation of Phosphodiester Oligodeoxynucleotides and Phosphorothioate Antisense Oligodeoxynucleotides by Capillary Zone Electrophoresis at a Low pH
β Scribed by LI, Q; CHEN, R; SUN, Y; HU, Y
- Book ID
- 122793836
- Publisher
- Chinese Electronic Periodical Services
- Year
- 2007
- Tongue
- English
- Weight
- 385 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1000-8713
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## Abstract Amino Acids are cations at low PH and can be readily separated by capillarty electrophoresis provided an alkanesulfonic acid is added to the elecrolyte carrier. Formation of a Positive net charge on the bare fusedβsilica surface at low PH was confirmed by measurement of an anodic electr
We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in