The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide- polyacrylamide conjugate
✍ Scribed by Takahisa Anada; Takumi Arisawa; Yoshihisa Ozaki; Tohru Takarada; Yoshiki Katayama; Mizuo Maeda
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 115 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a key factor in achieving efficient separation of oligodeoxynucleotides with the same chain length.