Separation of multiphosphorylated peptide isomers by CZE

✍ Scribed by Marika V. Muetzelburg; Ralf Hoffmann

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 485 KB
• Volume: 29
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200800089

✦ Synopsis

Abstract

A separation of mono, doubly and triply phosphorylated isomers was developed with CZE with an aqueous electrolyte containing 3.9 mol/L formic acid and 30% v/v trifluoroethanol. Thus a mixture of ten phosphopeptides corresponding to the human tau sequence 226–240 was separated within 70 min. Although peptides with different phosphorylation degrees, i.e. 0–3 phosphate groups, were well separated, some of the phosphopeptide isomers containing one or two phosphate groups were only partially separated. The electrolyte system is compatible with both MALDI‐ and ESI‐MS, allowing a direct coupling, and thus could have some interesting applications in proteomics.