Separation of large DNA molecules in alternating asymmetric electric fields
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 102 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0020-711X
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It has been reported that secondary pulsed field gel (SPFG) electrophoresis can dramatically increase the speed of separation of large DNA molecules without a decrease in resolution (Zhang, T. Y., Smith, C. L., and Cantor, C. R. (1991) Nucleic Acids Res. 19, \(1291-\) 1296). However, our attempts to
The electrophoresis of a series of DNA fragments ranging in size from 0.5 to 12 kilobase pairs, has been studied as a function of agarose gel concentration and electric field strength. The apparent mobility of all fragments decreased with decreasing electric field strength and with increasing gel co