Reorientation of large DNA molecules in concentrated polyacrylamide solution during crossed-field electrophoresis

Simulation of reduced band broadening du

Simulation of reduced band broadening during single-stranded DNA pulsed field electrophoresis in polyacrylamide gels

✍ Pascal Mayer; Dr. Gary W. Slater; Guy Drouin 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 816 KB

## Abstract Using a computer simulation algorithm based on the reptation model, we investigated the effects of pulsed field gel electrophoresis on the separation of single‐stranded DNA molecules in denaturing polyacrylamide gels. Pulsed fields that combine two different field intensities were found