Separation of keto—enol tautomers of chlortetracycline and 4-epichlortetracycline by liquid chromatography on poly(styrene—divinylbenzene)copolymer

✍ Scribed by Weng Naidong; E. Roets; R. Busson; J. Hoogmartens

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 456 KB
• Volume: 8
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(90)80137-e

✦ Synopsis

Keto and enol tautomers of chlortetracycline and 4-epichlortetracycline were successfully separated by low temperature high-performance liquid chromatography on poly(styrene-divinylbenzene)copolymer. The keto-enol tautomerism occurs between C-11a and C-12. The elucidation of the chemical structure of the tautomers was realized with the help of on-line and off-line UV-spectrophotometry and of 13C and 1H NMR spectrometry. The kinetics of the equilibrium reaction were investigated.