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Separation of DNA restriction fragments by ion-pair chromatography

✍ Scribed by Stig Eriksson; Gunnar Glad; Per-Åke Pernemalm; Elisabeth Westman

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
514 KB
Volume
359
Category
Article
ISSN
1873-3778

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✍ Yoshihiro Ohmiya; Yoichi Kondo; Toshihiko Kondo 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 925 KB

A nonporous QA column (a strong anion exchanger) was used for HPLC of DNA fragments. This column was successfully employed to separate small (ca. 10 bp) and intermediate size (ca. 10 kbp) DNA fragments from each other. The column also separated double-stranded DNA from its single-stranded form, and