Separation of DNA restriction fragments by high-performance ion-exchange chromatography on a non-porous ion exchanger
β Scribed by Yoshio Kato; Yosuke Yamasaki; Akane Onaka; Takashi Kitamura; Tustomu Hashimoto; Tomoaki Murotsu; Shinichi Fukushige; Kenichi Matsubara
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 338 KB
- Volume
- 478
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A nonporous QA column (a strong anion exchanger) was used for HPLC of DNA fragments. This column was successfully employed to separate small (ca. 10 bp) and intermediate size (ca. 10 kbp) DNA fragments from each other. The column also separated double-stranded DNA from its single-stranded form, and
A mixture of 12 angiotensins was separated by high-performance liquid chromatography on a weak anion-exchange bonded phase using a triethylammonium acetate buffer and acetonitrile as the eluant. An excellent separation of these compounds was obtained. Recoveries for all 12 were over 90%, as determin
The on-line coupling of high-performance anion-exchange chromatography (HPAEC) with ion spray mass spectrometry, for the analysis of neutral and acidic oligosaccharides, is described. On-line desalting is performed by a cation-exchange membrane system which replaces sodium for hydronium ions from th