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Separation of D- and L-amino acids by ion exchange column chromatography in the form of alanyl dipeptides

✍ Scribed by J. Csapó; I. Tóth-Pósfai; ZS. Csapó-Kiss


Publisher
Springer
Year
1991
Tongue
English
Weight
373 KB
Volume
1
Category
Article
ISSN
0939-4451

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Free amino acids (FAA) were separated by reversed-phase ion-pair liquid chromatography using a column-switching technique with isocratic elution. The eluted FAA were monitored by fluorescence detection using o-phthalaldehyde reagent. A 5-,um phenyl-bonded silica gel column (5 cm x 4.6 mm I.D.) was u