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Separation of free amino acids by reversed-phase ion-pair chromatography with column switching and isocratic elution

✍ Scribed by Mitsuko Hirukawa; Masako Maeda; Akio Tsuji; Toshihiko Hanai

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 446 KB
Volume: 507
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)84185-6

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✦ Synopsis

Free amino acids (FAA) were separated by reversed-phase ion-pair liquid chromatography using a column-switching technique with isocratic elution. The eluted FAA were monitored by fluorescence detection using o-phthalaldehyde reagent. A 5-,um phenyl-bonded silica gel column (5 cm x 4.6 mm I.D.) was used for the hydrophobic and basic amino acids, which were separated within 20 min. A 5-pm octadecyl-bonded silica gel column (25 cm x 4.6 mm I.D.) was used for polar acidic and small amino acids, which were separated within 11 min. The system was applied to the FAA analysis of hydrolysate of bovine serum albumin.

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