Separation of cyclodextrins and their derivatives by thin-layer and preparative column chromatography

✍ Scribed by Jindrich Jindrich; Josef Pitha; Bengt Lindberg

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 378 KB
• Volume: 275
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(95)00071-z

✦ Synopsis

Cyclodextrins, their derivatives and products of their partial hydrolysis are clearly separated by chromatography on silica gel by mobile phases containing aqueous ammonia and organic solvent (acetonitrile or 1-propanol). This system allows separation of compounds with different numbers of substituents on cyclodextrin, but does not allow the separation of isomers; the system can be used both for thin-layer chromatography and for preparative column chromatography. The isomers in the fraction thus obtained can be in some cases separated, after peracetylation, by chromatography on silica gel using a mobile phase consisting of methanol and dichloromethane. This chromatographic sequence potentially yields derivatives of cyclodextrins in quantities which enable evaluation of their solubilization and biological properties.