✦ LIBER ✦

Separation of Chiral Amino Acids by Micellular Electrokinetic Chromatography with Derivatized Cyclodextrins

✍ Scribed by Kapila DeSilva; Theodore Kuwana

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 86 KB
Volume: 11
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199707)11:4<230::aid-bmc678>3.0.co;2-2

No coin nor oath required. For personal study only.

✦ Synopsis

The chiral separation of several amino acid (AA) enantiomers derivatized with naphthalene-2,3-dialdehyde (NDA) was achieved by use of cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Both neutral hydroxypropyl ␤-cyclodextrin (HP-␤-CD) and charged carboxymethyl ␤-cyclodextrin (CM-␤-CD) were used as buffer additives for optical resolution of derivatized amino acids. The order of elution and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl suphate, CD and amino acid system. Furthermore, the importance of SDS for the separation of a mixture of AAs, and the effect of CD and analyte concentration on the resolution will be discussed.

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