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Separation of bacteria by sedimentation field-flow fractionation

✍ Scribed by Yu-Shu Gao; Stanley C. Lorbach; Robert Blake II

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 92 KB
Volume: 9
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1997)9:6<497::aid-mcs6>3.0.co;2-x

No coin nor oath required. For personal study only.

✦ Synopsis

Growth of the soil bacterium Stenotrophomonas maltophilia in the presence of selenite produced a mixture of two types of cells: those that contained numerous electron-dense bodies of elemental selenium and those that did not. Light diffraction and electrical impedance measurements indicated that the cells that contained visible bodies of elemental selenium were approximately 30% larger than the cells that exhibited no visible accumulation of selenium. Sedimentation field-flow fractionation was applied to a stationary-phase culture of bacteria growth in the presence of 2.0 mM selenite. Complete separation of selenium-free cells from those that contained elemental selenium was achieved at a field strength of 331 cmrs 2 .

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