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Separation of protein inclusion bodies from Escherichia coli lysates using sedimentation field-flow fractionation

✍ Scribed by S. Kim Ratanathanawongs Williams; Gregory M. Raner; Walther R. Ellis Jr.; J. Calvin Giddings


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
126 KB
Volume
9
Category
Article
ISSN
1040-7685

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✦ Synopsis


TRIBUTE TO PROFESSOR J. CALVIN GIDDINGS

I am very fortunate to have had the opportunity to work closely with Professor J. Calvin Giddings for the past 10 years. During this time, he has been both a mentor and a friend. The guidance and freedom that he has given me over the years have greatly impacted my professional growth and research directions. His insightful approaches to scientific problems, his thoroughness, and his good naturedness during times of stress are lessons that will always be remembered.

Cal Giddings approached recreational activities with the same zeal as he did work. Our skiing, river running, and mountain biking expeditions were both enjoyable and intense. The path that Cal inevitably took was the one where all others had failed or avoided because of its difficulty. Just as with work, there were no obstacles that were unsurmountable. They were challenges that could be overcome. Over the years, this has also become my personal philosophy.

I will miss him a great deal.


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Use of flow field-flow fractionation for
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Asymmetrical flow field-flow fractionation was and subunits due to the rather laborious experiments used for rapid (8-14 min) separation of ribosomes and and the experimental skill required (Birnbaum and their subunits. The amount of ribosomes and the mass Bailey, 1991). Moreover, artifacts occurrin