𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation of adult chains of abnormal haemoglobin: Identification by reversed-phase high-performance liquid chromatography

✍ Scribed by Paul Angoué Yapo; Jacques Y. Datté; Ayekoé Yapo; Henri Wachman

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
98 KB
Volume
18
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Rare abnormal haemoglobin (Hb) often poses a problem in the diagnosis of relative electrophoresis mobility. The rare neutral Hbs interact with the Hb S and thus can increase the severity of sickle cell anaemia. In the present study, we investigated the use of reversed‐phase high‐performance liquid chromatography (RP‐HPLC) as a method for the identification of neutral or unstable Hb, and compared it with the well‐known techniques urea‐triton electrophoresis (UT) and isoelectrofocusing (IEF). The results of our experiments showed that Bucuresti Hb was separated without ambiguity by RP‐HPLC. To date, no other electrophoresis techniques have been able to isolate this Hb. We also examined the Hb Nouakchott, which was the most hydrophobic Hb. Moreover, the adult and foetal chains of globins (especially the Aγ^t^ and Aγ^i^ chains) were quantified on the same chromatogram. J. Clin. Lab. Anal. 18:65–69, 2004. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Analysis of eleven capsaicinoids by reve
Analysis of eleven capsaicinoids by reversed-phase high performance liquid chromatography
✍ Marie-Noëlle Maillard; Pierre Giampaoli; Hubert M. J. Richard 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB 👁 2 views

More than 90 dierent capsicums are found all over the world. They contain capsaicinoids, which are responsible for the hot and pungent ¯avour. After extraction by a methanol±water mixture (60 : 40, v/v) from capsicum oleoresins, 13 compounds were separated by analytical HPLC on a C18 Hypersil column

Desalting of nucleotides by reverse-phas
Desalting of nucleotides by reverse-phase high-performance liquid chromatography
✍ Dexter B. Northrop; Colin J. Newton 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 165 KB

Chromatography of AMP, NAD+, or NADH on a reverse-phase C,, Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.