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Separation of Acidic Components of Vellozia flavicans by Silica Gel/Potassium Hydroxide Chromatography

✍ Scribed by Angelo C. Pinto; Octávio A. C. Antunes; Claudia M. Rezende; Carlos Roque D. Correia

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 112 KB
Volume: 8
Category: Article
ISSN: 0958-0344
DOI: 10.1002/(sici)1099-1565(199701)8:1<14::aid-pca326>3.0.co;2-6

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✦ Synopsis

The acidic compounds of the hexane extract of Vellozia flavicans could be selectively isolated when submitted to McCarthy and Duthie chromatography on a potassium hydroxide impregnated silica gel flash column followed by purification by C-18 reversed-phase semipreparative high pressure liquid chromatography. This method allowed the characterization of five acidic diterpenes, two of which could only be identified by co-injection of the appropriate standards owing to their very low concentration in the extract.

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