A method is described for the qualitative and quantitative determination of configurational isomers of zeaxanthin (= 3,3' -dihydroxy-P, p -carotene) and lutein (= 3 3 -dihydroxya-carotene). It is based on the reaction of these zeaxanthin and lutein isomers with (S)-(+)-e-(l -naphthyl) ethyl isocyana
Separation of (3S, 3′S)-, (3R 3′R)- and (3S, 3′R)-astaxanthin via (−)-camphanic acid esters
✍ Scribed by Vecchi, Max ;Müller, Robert K.
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 153 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A method is described for the qualitative and quantitative determination of configurational isomers of astaxanthin. It is based on the esterification of astaxanthin with (—)‐camphanic acid chloride and analysis of the corresponding diesters by HPLC.
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## Abstract The temperature dependent CD. spectra of (3__S__, 3′__R__)‐ and (3__S__, 3′__S__)‐adonixanthin are compared with those of (3__R__, 3′__R__)‐zeaxanthin (**1**) and (3__S__, 3′__S__)‐astaxanthin (**2**). The room temperature spectra of **1** and **2** are quite similar. On cooling to −180
Epoxy ring opening of 2 and 2 with [ l -1 4 C ] hexadecyl copper lo gave respectivezy t h e analog 4 of agaric acid trimethyl e s t e r and methy2 b -1 4 ~J a g a r i c a t e 5, which-was hydrolysed t o -& by Zithium hy-&oxide i n I, 2-dimethoxyethane (DME). Epoxy ring trans opening of -2 with d i