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Separation and quantitative analysis of O-linked phosphoamino acids by isocratic high-performance liquid chromatography of the 9-fluorenylmethyl chloroformate derivatives

โœ Scribed by Joseph S. Niedbalski; David P. Ringer


Book ID
107714373
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
671 KB
Volume
158
Category
Article
ISSN
0003-2697

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A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue o