An HPLC procedure for the isolation and quantitation of total and individual dolichyl esters in tissues has been developed. The purified lipid extracts are subjected to sequential reversed-phase, straight-phase, and reversed-phase HPLC, which yield complete resolution and high recovery of the indivi
Separation and quantitation of polyethylene glycols 400 and 3350 from human urine by high-performance liquid chromatography
β Scribed by Colleen M. Ryan; Martin L. Yarmush; Ronald G. Tompkins
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 364 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chrom
A sensitive, reliable method for the extraction, separation, and quantitation of methylprednisolone from rat brain is reported. The method can accurately quantitate methylprednisolone levels between 9.8 and 2500 ng/injection using a two-step HPLC separation and monitoring absorbance at 254 nm. A 90%
## Abstract A direct HPLC method for chiral separation of dorzolamide hydrochloride (4__S__,6__S__) and its enantiomer (4__R__,6__R__) was developed. Dorzolamide (4__S__,6__S__) and its antipode were separated on a chiralβΞ±~1~βacid glycoprotein column (150Γ4.0βmm, 5βΞΌm). The influences of pH, tempe
The common mobile phase hexane/isopropanol/water used for separation of phospholipids on highperformance liquid chromatography silica columns poses several problems, such as incomplete separation and rapid column deterioration. By inclusion of 5 mM ammonium sulfate in the aqueous phase, we were able