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Separation and purification of periplasmic cytochrome c553 using reversed micelles

โœ Scribed by Somnuk Jarudilokkul; Ludger H. Poppenborg; Francesca Valetti; Gianfranco Gilardi; David C. Stuckey

Book ID
110270852
Publisher
Springer-Verlag
Year
1999
Tongue
English
Weight
66 KB
Volume
13
Category
Article
ISSN
0951-208X

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โœ J. R. S. Alves; L. P. Fonseca; M. T. Ramalho; J. M. S. Cabral ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 466 KB

The extraction of penicillin acylase by reverse miccllar solutions of a surfactant was studied. A 50 mM solution of dioctyl sodium sulphosuccinatc in isooctane extracted 46 % of the enzyme activity in a crude pcriplasmic extract of induced cells of E. coli ATCC 9637. The increase in the specific act