Separation and purification of penicillin acylase fromEscherichia coliusing AOT reverse micelles

✍ Scribed by J. R. S. Alves; L. P. Fonseca; M. T. Ramalho; J. M. S. Cabral

• Book ID: 104644010
• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 466 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00151573

✦ Synopsis

The extraction of penicillin acylase by reverse miccllar solutions of a surfactant was studied. A 50 mM solution of dioctyl sodium sulphosuccinatc in isooctane extracted 46 % of the enzyme activity in a crude pcriplasmic extract of induced cells of E. coli ATCC 9637. The increase in the specific activity of the final enzyme preparation, after stripping of the organic phase at pH 7.5, in the prcscncc of 1 M KCI, was 8 -fold.